Rapid identification of Mycobacterium tuberculosis in cultures by molecular and immunological methods

Objective : The study aimed to evaluate molecular and immunological methods propose a workflow using them for tuberculosis (TB) diagnosis routine. Methods A cross-sectional retrospective was performed, including 121 liquid cultures from TB laboratory located in the extreme south of Brazil. All were positive Mycobacterium complex (MTBC) by in-house Polymerase Chain Reaction (PCR) DNA extracted CTAB method (PCR-CTAB) IS6110 detection. These subjected faster tests than this one, MPT64 assay PCR thermal lysis (PCR-TL), these evaluated MTBC identification PCR- as reference method. Results :The sensitivity PCR-TL identify PCR-CTAB 73.6% (89/121) 98.3% (119/121), respectively. We proposed based on use suggestive MTBC, case negative result, we suggest performance PCR-TL. is suggested only if are negative. Conclusions capable confirming should continue be standardized, tested, optimized meet ideal requirements simplicity, quickness, effectiveness. have differences execution detection cultures, but they rapid tools diagnosis.